Buffers
Citrate Buffer (10X) HIER Solution (pH 6.0) is designed to significantly enhance immunohistochemical staining with many commercially available primary antibodies. This is accomplished by unmasking the antigens and epitopes in formalin fixed and paraffin embedded tissue sections . All our buffers are specially formulated and filtered to ensure that they are of the highest possible quality, longest stability, and guaranteed to be free of contaminants.
Citrate Buffer (10x) pH 6.0
Citrate Buffer
C0001.1L
C0001.10L
C0001.20L
Description:
1L $99.00
10L $345.00
20L $590.00
Questions about product specifications and bulk discounts can be sent to:
1. Rehydrate tissue slides.
2. In a plastic Coplin jar, add 5 ml of Citrate Buffer and 45 ml of deionized water.
3. Loosely cap the coplin jar and place in a vegetable steamer for 15 minutes to heat solution (Prior to submersion
4. Using tongs, remove the coplin jar from the steamer. Carefully remove cap and submerge slides. Recap loosely and return jar to steamer.
5. Steam for 20 minutes. Allow jar with solution and slides to cool to room temperature. 6. Remove slides and rinse in buffer several times.
7. Continue with staining according to protocol.
2. In a plastic Coplin jar, add 5 ml of Citrate Buffer and 45 ml of deionized water.
3. Loosely cap the coplin jar and place in a vegetable steamer for 15 minutes to heat solution (Prior to submersion
4. Using tongs, remove the coplin jar from the steamer. Carefully remove cap and submerge slides. Recap loosely and return jar to steamer.
5. Steam for 20 minutes. Allow jar with solution and slides to cool to room temperature. 6. Remove slides and rinse in buffer several times.
7. Continue with staining according to protocol.
Instruction for Use:
Spec Sheet
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